single-cell portal database Search Results


single cell sequencing  (Broad Clinical Labs)
96
Broad Clinical Labs single cell sequencing
Single Cell Sequencing, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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single cell portal database  (Broad Institute Inc)
90
Broad Institute Inc single cell portal database
Expression of CSF ligands in murine glioma and human GBM: ( A ) Murine KR158B gliomas express the CSF ligands M-CSF and IL-34 but not GM-CSF. KR158B cells were plated at different numbers (50, 100, 200 thousand) in 24 well plates and the conditioned media were subjected to ELISA after 24 h for cytokine quantification. CSF1R ligands, M-CSF and IL-34, were found at a higher concentration in the media compared to GM-CSF (n = 3 experiments). ( B ) Human GBM expression of CSF ligands was determined from a search of the OncoDB <t>database.</t> TCGA dataset was queried for GBM gene expression. Results were compared to normal brain tissue from GTEx. CSF-1 (M-CSF) was differentially upregulated in the GBM microenvironment (n = 148 patients) compared to normal brain (n = 200 patients). CSF-1 was found at the highest level compared to the other CSF ligands. ( C ) <t>Single-Cell</t> <t>Portal</t> database from Broad Institute was accessed for evaluation of CSF ligand expression. The “Programs, Origins, and Niches of Immunomodulatory Myeloid Cells in Human Gliomas” dataset was used for analysis. Multiple human tumors (85) and cells (515,782) were analyzed. CSF-1 and IL34 are expressed by the malignant cell population. CSF-2 and CSF-3 (GM-CSF and G-CSF) were lowly expressed. Students’ t -test statistical analysis was conducted. Differences are compared to the stimulated control condition. p -values: >0.05 (ns), 0.0332 (*), 0.0002 (***), <0.0001 (****).
Single Cell Portal Database, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single cell portal database/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
single cell portal database - by Bioz Stars, 2026-03
90/100 stars
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single cell beta portal database  (Broad Institute Inc)
90
Broad Institute Inc single cell beta portal database
Expression of CSF ligands in murine glioma and human GBM: ( A ) Murine KR158B gliomas express the CSF ligands M-CSF and IL-34 but not GM-CSF. KR158B cells were plated at different numbers (50, 100, 200 thousand) in 24 well plates and the conditioned media were subjected to ELISA after 24 h for cytokine quantification. CSF1R ligands, M-CSF and IL-34, were found at a higher concentration in the media compared to GM-CSF (n = 3 experiments). ( B ) Human GBM expression of CSF ligands was determined from a search of the OncoDB <t>database.</t> TCGA dataset was queried for GBM gene expression. Results were compared to normal brain tissue from GTEx. CSF-1 (M-CSF) was differentially upregulated in the GBM microenvironment (n = 148 patients) compared to normal brain (n = 200 patients). CSF-1 was found at the highest level compared to the other CSF ligands. ( C ) <t>Single-Cell</t> <t>Portal</t> database from Broad Institute was accessed for evaluation of CSF ligand expression. The “Programs, Origins, and Niches of Immunomodulatory Myeloid Cells in Human Gliomas” dataset was used for analysis. Multiple human tumors (85) and cells (515,782) were analyzed. CSF-1 and IL34 are expressed by the malignant cell population. CSF-2 and CSF-3 (GM-CSF and G-CSF) were lowly expressed. Students’ t -test statistical analysis was conducted. Differences are compared to the stimulated control condition. p -values: >0.05 (ns), 0.0332 (*), 0.0002 (***), <0.0001 (****).
Single Cell Beta Portal Database, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single cell beta portal database/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
single cell beta portal database - by Bioz Stars, 2026-03
90/100 stars
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Expression of CSF ligands in murine glioma and human GBM: ( A ) Murine KR158B gliomas express the CSF ligands M-CSF and IL-34 but not GM-CSF. KR158B cells were plated at different numbers (50, 100, 200 thousand) in 24 well plates and the conditioned media were subjected to ELISA after 24 h for cytokine quantification. CSF1R ligands, M-CSF and IL-34, were found at a higher concentration in the media compared to GM-CSF (n = 3 experiments). ( B ) Human GBM expression of CSF ligands was determined from a search of the OncoDB database. TCGA dataset was queried for GBM gene expression. Results were compared to normal brain tissue from GTEx. CSF-1 (M-CSF) was differentially upregulated in the GBM microenvironment (n = 148 patients) compared to normal brain (n = 200 patients). CSF-1 was found at the highest level compared to the other CSF ligands. ( C ) Single-Cell Portal database from Broad Institute was accessed for evaluation of CSF ligand expression. The “Programs, Origins, and Niches of Immunomodulatory Myeloid Cells in Human Gliomas” dataset was used for analysis. Multiple human tumors (85) and cells (515,782) were analyzed. CSF-1 and IL34 are expressed by the malignant cell population. CSF-2 and CSF-3 (GM-CSF and G-CSF) were lowly expressed. Students’ t -test statistical analysis was conducted. Differences are compared to the stimulated control condition. p -values: >0.05 (ns), 0.0332 (*), 0.0002 (***), <0.0001 (****).

Journal: Cancers

Article Title: CSF1R Ligands Expressed by Murine Gliomas Promote M-MDSCs to Suppress CD8 + T Cells in a NOS-Dependent Manner

doi: 10.3390/cancers16173055

Figure Lengend Snippet: Expression of CSF ligands in murine glioma and human GBM: ( A ) Murine KR158B gliomas express the CSF ligands M-CSF and IL-34 but not GM-CSF. KR158B cells were plated at different numbers (50, 100, 200 thousand) in 24 well plates and the conditioned media were subjected to ELISA after 24 h for cytokine quantification. CSF1R ligands, M-CSF and IL-34, were found at a higher concentration in the media compared to GM-CSF (n = 3 experiments). ( B ) Human GBM expression of CSF ligands was determined from a search of the OncoDB database. TCGA dataset was queried for GBM gene expression. Results were compared to normal brain tissue from GTEx. CSF-1 (M-CSF) was differentially upregulated in the GBM microenvironment (n = 148 patients) compared to normal brain (n = 200 patients). CSF-1 was found at the highest level compared to the other CSF ligands. ( C ) Single-Cell Portal database from Broad Institute was accessed for evaluation of CSF ligand expression. The “Programs, Origins, and Niches of Immunomodulatory Myeloid Cells in Human Gliomas” dataset was used for analysis. Multiple human tumors (85) and cells (515,782) were analyzed. CSF-1 and IL34 are expressed by the malignant cell population. CSF-2 and CSF-3 (GM-CSF and G-CSF) were lowly expressed. Students’ t -test statistical analysis was conducted. Differences are compared to the stimulated control condition. p -values: >0.05 (ns), 0.0332 (*), 0.0002 (***), <0.0001 (****).

Article Snippet: Single Cell Portal database from Broad Institute was accessed for evaluation of CSF ligand expression.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Gene Expression, Control